Differential gene expression of chemokines in KRAS and BRAF mutated colorectal cell lines: Role of cytokines

Figure 8 The figure shows the results of KRAS inhibition from transient transfection of KRAS siRNA in DLD-1 and Caco2 cell lines at RNA and protein level. A-D: Relative expression of KRAS at RNA-level by RT-PCR (A and B) and protein expression by Western blotting (C and D) at 48 h and 72 h after KRAS inhibition; E, F: Note the significant down-regulation of KRAS at 48 and 72 h at protein level by densitometric analysis in both, DLD1 and Caco2 cell-lines. Changes are shown in percent, compared to scrambled siRNA. Data are presented as mean ± SE of 3 independent experiments with double confirmation. ^aP < 0.05 vs scrambled 72 h.