Differential gene expression of chemokines in KRAS and BRAF mutated colorectal cell lines: Role of cytokines

Figure 1 Basal mRNA expression of acute-phase cytokines in different intestinal epithelial cell-lines. A: Tumor necrosis factor-α (TNF-α); B: Interleukin (IL-β); C: Interferon-γ (IFN-γ); D: Interleukin-6 (IL-6). The mutation is given in parenthesis. 5 × 10⁵ cells were plated into 6 well plates and grown for 24 h. The cells were harvested, total RNA was isolated and first strand cDNA was prepared from 1 μg of total RNA. Ct values were normalized to β-actin as a housekeeping gene. The results were compared with the fold changes of Caco2 mRNA expression, taken as a control. Results represent mean ± SE (^aP < 0.05 vs Caco2 analyzed by one way ANOVA, n = 4).