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©2014 Baishideng Publishing Group Co.
World J Gastroenterol. Mar 14, 2014; 20(10): 2688-2694
Published online Mar 14, 2014. doi: 10.3748/wjg.v20.i10.2688
Published online Mar 14, 2014. doi: 10.3748/wjg.v20.i10.2688
Table 1 Primers and probes used for genotyping CD14-260C/T and -651C/T
| Polymorphism | Primers and probes | Length, bp |
| -260 C/T | 5'-CACCCACCAGAGAAGGCTTA-3' | 212 |
| 5'-ATCACCTCCCCACCTCTCTT-3' | ||
| Common probe | 5'-CCCCCTCCCTGAAACATCCTTTTTTTTTTTTTTTTTTT-FAM-3' | |
| Discriminating | 260C/T-R_G | 77 |
| probes | 5'-TTTTTTTTTTTTTTTTTGCAGAATCCTTCCTGTTACGGC | |
| 260C/T-R_A-3' | 79 | |
| 5'-TTTTTTTTTTTTTTTTTTTGCAGAATCCTTCCTGTTACGGT-3' | ||
| -651C/T | 5'-GGGTAGAATTAGGTTCAAG-3' | 103 |
| 5'-CTTAATCAAAGGAGCAAGG-3' | 1 | |
| Common probe | 5'-GTCTAAAGAAAAATTCCCCCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-FAM-3' | |
| Discriminating probes | 651C/T_C | 150 |
| 5'-TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGGTTCAAGAAAAGGAAGTTG-3' | ||
| 651C/T_T | 152 | |
| 5'-TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGGTTCAAGAAAAGGAAGTTA-3' |
- Citation: Li K, Dan Z, Hu XJ, Gesang LB, Ze YG, Bianba ZX, Ciren CM, Nie YQ. Association of CD14/-260 polymorphism with gastric cancer risk in Highland Tibetans. World J Gastroenterol 2014; 20(10): 2688-2694
- URL: https://www.wjgnet.com/1007-9327/full/v20/i10/2688.htm
- DOI: https://dx.doi.org/10.3748/wjg.v20.i10.2688