Experimental Papers
Copyright ©The Author(s) 1996.
World J Gastroenterol. Dec 15, 1996; 2(4): 194-196
Published online Dec 15, 1996. doi: 10.3748/wjg.v2.i4.194
Table 1 Assay of human fetal hepatic stimulating substance activity by 3H-TdR incorporation method
SampleSample volume (μL)Concentration of protein (mg/mL)cpm (mean ± SD)ISSpecific activity (IS/mg protein)Fold of purification
Control (0.9% NaCl)25-1275 ± 203---
Homogenization2552.6003162 ± 3292.481.891.00
ETOH-ppt hHSS258.2803683 ± 4013.0314.647.74
DEAE A50 peak V253.4702920 ± 2512.2926.4013.97
Nondenaturing PAGE major band250.1692039 ± 1141.60326.89172.76