Corticotropin-releasing factor receptor subtype 2 in human colonic mucosa: Down-regulation in ulcerative colitis

Figure 3 Comparison of corticotropin-releasing factor 2 receptor immunoreactivity in distal/sigmoid biopsies from healthy human subjects and patients with moderate ulcerative colitis and patients in remission. Tissue sections from mucosal biopsies were stained by immunofluorescence using specific antisera 2064a- corticotropin-releasing factor 2 (CRF₂) (A, C, E). Immunoreactivity for CRF₂ observed in the epithelial cells of the intestinal crypts in the control group, was not found in the UCact and UCrem groups. Replacement of the antiserum by non-specific rabbit IgG (B, D, F) abolished all specific staining. Original magnification 100×.