Inhibition of pacemaker activity in interstitial cells of Cajal by LPS via NF-κB and MAP kinase

Figure 7 Effects of mitogen-activated protein kinases inhibitors on (±)-S-nitroso-N-acetylpenicillamine-induced action in interstitial cells of Cajal. A: Pacemaker currents of interstitial cells of Cajal (ICCs) treated with 100 μmol/L SNAP at a holding potential of -70 mV; B: Pacemaker currents after pretreatment with PD 98059 (10 μmol/L) prior to SNAP treatment; C: Pacemaker currents after pretreatment with SB 203580 (10 μmol/L) prior to SNAP treatment; D: Pacemaker currents after pretreatment with L-NAME (10 μmol/L) prior to prostaglandin E2 (PGE₂) (5 μmol/L) treatment. The effects of mitogen-activated protein kinases inhibitors on the (±)-S-nitroso-N-acetylpenicillamine-induced action are summarized in E and F. Bars represent mean ± SE values (n = 5 per group). Con: Control, PD: PD 98059, SB: SB203580; SNAP: S-nitroso-N-acetylpenicillamine; L-NAME: NG-Nitro-L-arginine Methyl Ester.