Inhibition of pacemaker activity in interstitial cells of Cajal by LPS via NF-κB and MAP kinase

Figure 5 Effects of Mitogen-activated protein kinases inhibitors on lipopolysaccharide-induced action in interstitial cells of Cajal. A: Pacemaker currents of interstitial cells of Cajal (ICCs) incubated at 37  °C with 200 μg/mL of lipopolysaccharide (LPS) for 12 h at a holding potential of -70 mV; B: Pacemaker currents after pretreatment with PD 98059 (10 μmol/L) for 2 h prior to LPS incubation; C: Pacemaker currents after pretreatment with SB 203580 (10 μmol/L) for 2 h prior to LPS incubation; D: Pacemaker currents after pretreatment with c-Jun N-terminal kinase (JNK) inhibitor II (10 μmol/L) for 2 h prior to LPS incubation. The effects of mitogen-activated protein kinases inhibitors on the LPS-induced action are summarized in E and F. Bars represent mean ± SE values (n = 7 per group). ^aP < 0.05 vs LPS.