Inhibition of pacemaker activity in interstitial cells of Cajal by LPS via NF-κB and MAP kinase

Figure 4 Effects of SN-50 or actinomycin D on lipopolysaccharide-induced action in interstitial cells of Cajal. A: Pacemaker currents of interstitial cells of Cajal (ICCs) incubated at 37 °C with 200 μg/mL of lipopolysaccharide (LPS) for 12 h at a holding potential of -70 mV; B: Pacemaker currents in ICCs pretreated with SN-50 (10 μmol/L) for 2 h prior to LPS incubation; C: Pacemaker currents in ICCs pretreated with actinomycin D (10 μmol/L) for 2 h prior to LPS incubation. The effects of SN-50 or actinomycin D on the LPS-induced action in ICCs are summarized in D and E. Bars represent mean ± SE values (n = 5 per group). ^aP < 0.05 vs LPS alone treatment. SN: SN-50, AD: Actinomycin D.