Inhibition of pacemaker activity in interstitial cells of Cajal by LPS via NF-κB and MAP kinase

Figure 2 Expression of TLR4, iNOS and COX-2 in c-Kit positive cells. A: Double labeling of TLR4- and c-Kit-like immunoreactivity in cultured interstitial cells of Cajal (ICCs). Green (c-Kit) and red (TLR4) result in the mixed color yellow, indicating the colocalization of both peptides; B: Double labeling of iNOS and c-Kit-like immunoreactivity in ICCs. Green (c-Kit) and red (iNOS) result in the mixed color yellow, indicating the colocalization of both peptides; C: Double labeling of COX-2 and c-Kit-like immunoreactivity in ICCs. Green (c-Kit) and red (COX-2) result in the mixed color yellow, indicating the colocalization of both peptides (bar = 20 μm).