Long interspersed nuclear element ORF-1 protein promotes proliferation and resistance to chemotherapy in hepatocellular carcinoma

Figure 5 Long interspersed nuclear element ORF-1 protein modulates the expression of genes related to cell proliferation and apoptosis regulation. A: HepG2 cells were transfected with the fludarabine + high-dose cytarabine + G-CSF human long interspersed nuclear element-1 (FLAG-LINE-1) ORF-1p vector, or the empty vector. The assays were performed three times with similar results. ^aP < 0.05 vs FLAG; B: HepG2 cells were transfected with control siRNA or LINE-1 ORF-1p siRNA. The bar graph shows the relative protein expression level of each protein compared with GAPDH. The assays were performed three times with similar results. ^aP < 0.05 vs FLAG; ^cP < 0.05 vs control siRNA. GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.