Long interspersed nuclear element ORF-1 protein promotes proliferation and resistance to chemotherapy in hepatocellular carcinoma

Figure 4 Long interspersed nuclear element ORF-1 protein regulates the transcription of Luciferase reporters. HepG2 cells were transfected with the fludarabine + high-dose cytarabine + G-CSF human long interspersed nuclear element-1 (FLAG-LINE-1) ORF-1p or FLAG empty vector, LINE-1 ORF-1p siRNA or control siRNA. HepG2 cells were co-transfected with Luc-p21 (A), Luc-SBE (B), Luc-Sp1 (C), or Luc-ARE (D). The assays were performed three times with similar results. ^aP < 0.05 vs FLAG; ^cP < 0.05 vs control siRNA. GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.