Long interspersed nuclear element ORF-1 protein promotes proliferation and resistance to chemotherapy in hepatocellular carcinoma

Figure 3 Long interspersed nuclear element ORF-1 protein promotes apoptosis and anchor-independent growth of HepG2 cells. HepG2 cells were transfected with the empty vector or f ludarabine + high-dose cytarabine + G-CSF human long interspersed nuclear element-1 (FLAG-LINE-1) ORF-1p, or with the control siRNA or LINE-1 ORF-1p siRNA. A, B: The rates of apoptosis are shown in the figures (A). The assays were performed three times with similar results (B); C, D: Colony numbers shown in the photographs (C), or mean ± SD (n = 3) of triplicate independent experiments (D). ^aP < 0.05 vs FLAG; ^cP < 0.05 vs control siRNA; ^eP < 0.05 vs FLAG-LINE-1 ORF-1p. GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.