Long interspersed nuclear element ORF-1 protein promotes proliferation and resistance to chemotherapy in hepatocellular carcinoma

Figure 2 Long interspersed nuclear element ORF-1 protein promotes the proliferation of hepatocellular carcinoma cells. A: HepG2 cells were stably transfected with the fludarabine + high-dose cytarabine + G-CSF human long interspersed nuclear element-1 (FLAG-LINE-1) ORF-1p or the empty vector; B: HepG2 cells were stably transfected with the control siRNA or LINE-1 ORF-1p siRNA; C: BEL-7402 cells were stably transfected with the FLAG-LINE-1 ORF-1p or the empty vector; D: BEL-7402 cells were stably transfected with the control siRNA or LINE-1 ORF-1p siRNA; E: SMMC-7721 cells were stably transfected with the FLAG-LINE-1 ORF-1p or the empty vector; F: SMMC-7721 cells were stably transfected with the control siRNA or LINE-1 ORF-1p siRNA. The relative cell numbers (A_{490 nm}) were determined by MTT assays, relative cell numbers (A, B) with mean ± SD (n = 3). ^aP < 0.05 vs FLAG; ^cP < 0.05 vs control siRNA. GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.