Long interspersed nuclear element ORF-1 protein promotes proliferation and resistance to chemotherapy in hepatocellular carcinoma

Figure 1 Effect of long interspersed nuclear element ORF-1 protein on epirubicin, cisplatin and paclitaxel cytotoxicity. HepG2 cells were treated with the indicated amounts of epirubicin (A, B), cisplatin (C, D) or paclitaxel (E, F): 0 nmol/L, 0.5 nmol/L, 1 nmol/L, 2 nmol/L, 5 nmol/L, 10 nmol/L, 20 nmol/L, 50 nmol/L, 60 nmol/L. A: HepG2 cells were transfected with the fludarabine + high-dose cytarabine + G-CSF human long interspersed nuclear element-1 (FLAG-LINE-1) ORF-1p expression vector, or the empty vector; B: HepG2 cells were transfected with the control siRNA vector or the LINE-1 ORF-1p siRNA vector; C: HepG2 cells were transfected with the FLAG-LINE-1 ORF-1p expression vector, or the empty vector; D: HepG2 cells were transfected with the control siRNA vector or the LINE-1 ORF-1p siRNA vector; E: HepG2 cells were transfected with the FLAG-LINE-1 ORF-1p expression vector, or the empty vector; F: HepG2 cells were transfected with the control siRNA vector or the LINE-1 ORF-1p siRNA vector. The assays were performed three times with similar results. ^aP < 0.05 vs control. 1: Control; 2: Epirubicin; 3: Cisplatin; 4: Paclitaxel. GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.