Pseudomonas fluorescens-like bacteria from the stomach: A microbiological and molecular study

Table 1 Primers used to study Helicobacter pylori and Pseudomonas fluorescens isolates

Target gene	Primer name	Primer sequence (5’-3’)	PCR condition (number of cycles, size of product)
16S rRNA	16S F	TTGGAGAGTTTGATCCTGGCC	94 °C, 30 s; 59 °C, 30 s; 72 °C, 30 s (30, 1155 bp)
	16S R	ACGTCATCCCACCTTCTC
HSP60
Primary	HSP1	AAGGCATGCAATTTGATAGAGGCT	94 °C, 30 s; 56 °C, 30 s; 72 °C, 30 s (35, 594 bp)
	HSP2	CTTTTTTCTCTTTCATTTCCACTT
Nested	HSPN1	TTGATAGAGGCTACCTCTCC	94 °C, 30 s; 56 °C, 30 s; 72 °C, 30 s (35, 501 bp)
	HSPN2	TGTCATAATCGCTTGTCGTGC
Putative membrane-bound protein (PFMP)
Primary	PFMPF	TCTKRYCRMGAATCRARACWRYC	94 °C, 1 min; 50 °C, 1 min; 72 °C, 1 min (35, 704 bp)
	PFMPR	GKTWYTGCKCRWWKCSYTSMMC
Nested	PFMPNF	TGCGYWMMWCCYWRWCCWTGA	94 °C, 1 min; 50 °C, 1 min; 72 °C, 1 min (35, 613 bp)
	PFMPNR	AKCABGGTSCWGCMVRCCRBGC
mupV
Primary	mupVF	TGAGTTCGATGTGACCTGCCTG	94 °C, 1 min; 55 °C, 1 min; 72 °C, 1 min (35 cycles, 722 bp)
	mupVR	AACTCGCCAGATTGTCGTACAC
Nested	mupVnF	CAGCATTATCCTGCCACTGAC	94 °C, 1 min; 55 °C, 1 min; 72 °C, 1 min (35, 611 bp)
	mupVnR	ATGATGTCCTGGCACACCTGATC

PCR: Polymerase chain reaction.