Current trends in the development and application of molecular technologies for cancer epigenetics

Table 2 A summary of selected evidence for effects of butyrate in histone modification and histone modification assays in cancer cell culture models

Dietary component	Cell culture model	Observations	Histone modification assay	Ref.
Sodium butyrate	SW620 human colon carcinoma cells	Increased global histone H4 acetylation	Western blot	[70]
Sodium butyrate	A375 human melanoma and S91 mouse melanoma	Increased global histone H4 acetylation	Western blot	[98]
Sodium butyrate	Colo-320 human colon cancer cells	Increased acetylation of histone H3 and H4 within CDKN1A promoter site	ChIP	[64]
Sodium butyrate	EBC-1 human lung epithelial cells	Increased histone H3 and H4 acetylation associated with promoter of cathelicidin	ChIP	[99]
Sodium butyrate	HepG2 human hepatocarcinoma	Increased global histone H3 and H4 acetylation; Genome-wide changes in acetylation of DNA-bound histones	Western blot; ChIP-chip (ChIP and microarray hybridization)	[100]

CDKN1A: Cyclin-dependent kinase inhibitor 1A; ChIP: Chromatin immunoprecipitation.