Interference of suppressor of cytokine signaling 3 promotes epithelial-mesenchymal transition in MHCC97H cells

Figure 4 Effect of suppressor of cytokine signaling 3 small-interfering RNA on expression of epithelial-mesenchymal transition-associated molecules in MHCC97H cells. The cells were transiently transfected with negative control (NC) small-interfering RNA (siRNA) or suppressor of cytokine signaling 3 (SOCS3) siRNA for 48 h. A: Protein expression and mRNA levels of SOCS3 in MHCC97H cells were detected by Western blotting; B: Reverse transcription-polymerase chain reaction (RT-PCR) and quantitative real-time PCR; C: Protein expression and mRNA levels of epithelial-mesenchymal transition-associated molecules in MHCC97H cells were analyzed by Western blotting; D: RT-PCR and quantitative real-time PCR after normalization to β-actin mRNA. Data are presented as the mean ± SD from three independent experiments. ^aP < 0.05, ^bP < 0.01 vs NC siRNA.