Accommodation and peristalsis are functional responses to obstruction in rat hypertrophic ileum

Figure 5 Cellular localization of 5-HT3 receptors in control and hypertrophic ileum. Confocal images (0.5 μm sections; scale bar 20 μm) of myenteric plexus neurons isolated from control and hypertrophic ileal tissues. A: Image showing 5-HT3 receptor immunoreactivity visualized using donkey-anti-rabbit immunoglobulin G (IgG) conjugated with AlexaFluor 488 (green); B: Image showing clathrin immunoreactivity visualized using a donkey-anti-mouse IgG conjugated with Dylight 549 (red); C: Merged images from A and B; D: Image showing 5-HT3 receptor immunoreactivity visualized using donkey-anti-rabbit IgG conjugated with AlexaFluor 488 (green); E: Image showing clathrin immunoreactivity visualized using a donkey-anti-mouse IgG conjugated with Dylight 549 (red); F: Merged images from D and E.