Interleukin-17 plays a critical role in the acute rejection of intestinal transplantation

Figure 5 Expression of interleukin-17 in rat intestine and serum after transplantation. The intestines from inbred F344 were transplanted to LEW rats. The recipient rats were randomly divided into control and anti-interleukin (IL)-17 monoclonal antibody (mAb) treated groups. The rats were sacrificed on the 1^st, 2^nd, 3^rd, 5^th, and 7^th d after transplantation. A: Western blot analysis of graft proteins of different groups. β-actin were considered as an internal control; B: The bands were scanned and the protein expressions were analyzed with Band Scan software; C: Blood from the heart was extracted and the serum IL-17 levels was detected by enzyme-linked immunosorbent assay; D: The mRNA of IL-17 was detected by quantitative reverse transcriptase polymerase chain reaction. β-actin was considered as an internal control. ^aP < 0.05, ^bP < 0.01 vs control group.