Effectiveness of a hydroxynaphthoquinone fraction from Arnebia euchroma in rats with experimental colitis

Figure 6 Hydroxynaphthoquinone mixture decreases tumor necrosis factor-α expression in colonic tissue of rats with 2,4,6-trinitrobenzene sulfonic acid-induced colitis. A: Tumor necrosis factor (TNF)-α protein bands; B: Integrated optical density (A) values of protein bands. Colitis was induced by intracolonic administration of TNBS (80 mg/kg, dissolved in 50% ethanol). Rats were treated daily for 7 d with HM (2.5, 5, 10 mg/kg) 24 h after TNBS instillation. Protein extracts were obtained from colons and TNF-α expression level was detected by Western blotting analysis. Lane 1: Controls; lane 2: Rats treated with TNBS alone; lane 3-5: Rats treated with TNBS plus HM (2.5, 5, 10 mg/kg). Data are represented as mean ± SD of 4 animals of each group. ^aP < 0.05 vs control; ^cP < 0.05 vs TNBS alone. HM: Hydroxynaphthoquinone mixture; TNBS: 2,4,6-trinitrobenzene sulfonic acid.