Effectiveness of a hydroxynaphthoquinone fraction from Arnebia euchroma in rats with experimental colitis

Figure 5 Immunostaining for tumor necrosis factor-α. A: Immunostaining sections; B: Integrated optical density (A) values of representative colonic samples of each group. Colitis was induced by intracolonic administration of TNBS (80 mg/kg, dissolved in 50% ethanol). Rats were treated daily for 7 d with HM (2.5, 5, 10 mg/kg) 24 h after TNBS instillation. Colons were excised and stained with anti-TNF-α antibody. 1: Control; 2: Rat treated with TNBS alone; 3-5: Rats treated with TNBS plus HM (2.5, 5, 10 mg/kg). Data are represented as mean ± SD of 4 animals of each group. ^aP < 0.05 vs control; ^dP < 0.01 vs TNBS alone. HM: Hydroxynaphthoquinone mixture (original magnification, × 400); TNBS: 2,4,6-trinitrobenzene sulfonic acid; TNF-α: Tumor necrosis factor-α.