Anti-miRNA-221 sensitizes human colorectal carcinoma cells to radiation by upregulating PTEN

Figure 5 Effects of anti-miR-221 on irradiation-induced death in Caco2 cells. The percentages of necrotic and apoptotic cells were measured by calculating the ratio of the number of corresponding cells to the number of total cells. A: Blank control; B: Caco2 cells transfected with negative control; C: Caco2 cells transfected with anti-miR-221; D: Caco2 cells under irradiation; E: Caco2 cells transfected with negative control under irradiation; F: Caco2 cells transfected with anti-miR-221 under irradiation. The right upper quadrant (FITC⁺/PI⁺) shown as necrotic cells. The right lower quadrant (FITC⁺/PI^-) shown as apoptotic cells; G: The status of cell death was determined by flow cytometry. Groups A-F are described as above. n = 3, mean ± SD; ^bP < 0.01 vs blank or negative control group. ^dP < 0.01 vs sole anti-miR-221 or irradiation group.