Anti-miRNA-221 sensitizes human colorectal carcinoma cells to radiation by upregulating PTEN

Figure 3 Effects of X-ray dose on miR-221 and phosphatase and tensin homolog deleted on chromosome 10 expression in colorectal carcinoma-derived cells. A: Expression of miR-221 was detected by real-time RT-PCR. The miR-221 expression level improved in a dose-dependent manner with the increase in radiation dose, ^bP < 0.01 vs control group; B: Expression of phosphatase and tensin homolog deleted on chromosome 10 (PTEN) protein was detected by Western blotting, β-actin was used as a housekeeping gene to normalize PTEN protein expression. The relative PTEN protein levels normalized against β-actin are shown at the top of each panel. The expression of PTEN protein was decreased in a dose-dependent manner with the increase in radiation dose; C: Expression of PTEN mRNA was detected by real-time RT-PCR. No significant changes in the PTEN mRNA levels were observed in the cells exposed to irradiation. The results are representative of three independent experiments.