Transforming growth factor-β and toll-like receptor-4 polymorphisms are not associated with fibrosis in haemochromatosis

Table 2 Polymerase chain reaction profiles for candidate gene high resolution melt analysis

Gene	Annealing temperature	Extension temperature	Melt analysis range
MCP-1	58 °C	72 °C	74 to 84 °C
CCR-2	58 °C	72 °C	74 to 84 °C
IL10-592	57 °C	72 °C	75 to 85 °C
IL10-1082	59 °C	72 °C	70 to 81 °C
TGF-β	65 °C (20 s)	72 °C (20 s)	79 to 89 °C
TLR4 299	57 °C	68 °C	66 to 78 °C
TLR4 399	58 °C	70 °C	69 to 81 °C
hOGG1	57 °C	68 °C	78 to 88 °C

MCP-1: Monocyte chemoattractant protein 1; CCR-2: Chemokine C-C motif receptor; IL10: Interleukin-10; TGF-β: Transforming growth factor-beta; TLR4: Toll-like receptor 4; hOGG1: Human 8-oxoguanine DNA glycosylase.