Synergistic effect of interleukin-10-receptor variants in a case of early-onset ulcerative colitis

Figure 4 Effects of mesalazine and azathioprine on inflammatory bowel diseases and familial adenomatous polyposis primary fibroblasts. A: Real time polymerase chain reaction (PCR) quantification analysis of interleukin-10 (IL10) mRNA; Real time RT-PCR quantification analysis was performed for IL10RB mRNA on primary fibroblasts extracted from an inflammatory bowel diseases (IBD) and a familial adenomatous polyposis (FAP) patient and incubated with mesalazine and azathioprine; B: Western blotting assay of tumour necrosis factor α receptors-I (TNFRI) and TNFRII and tumour necrosis factor α (TNFα) performed on protein extracts from primary fibroblasts of an IBD and of a FAP patient. IBD-1: Protein extract of the IBD proband primary fibroblasts incubated with 0.1% DMSO only; IBD-2: Protein extract of the IBD proband primary fibroblasts incubated with 0.1% DMSO and mesalazine; IBD-3: Protein extract of the IBD proband primary fibroblasts incubated with 0.1% DMSO and azathioprine; FAP-1: Protein extract of the FAP patient’s primary fibroblasts incubated with 0.1% DMSO only; FAP-2: Protein extract of the FAP patient’s primary fibroblasts incubated with 0.1% DMSO and mesalazine; FAP-3: Protein extract of the FAP patient’s primary fibroblasts incubated with 0.1% DMSO and azathioprine.