Clinicopathological and biological significance of cripto overexpression in human colon cancer

Figure 4 Effects of S1 siRNA on migration and invasion of colon cancer LS-174T cells. The LS-174T colon cancer cell line was used in this experiment. Cell migration was assessed in BioCoat control cell culture chambers. Transwell migration and invasion assays were performed using LS-174T cells cultured in 12-well plates containing either Matrigel-coated inserts (invasion assays) or 8 μm pore Biocoat^® control inserts (migration assays), according to the manufacturer’s instructions. Control, scrambled siRNA-, and S1 siRNA-treated cells were added to control and Matrigel chambers. A: The percentage of invasion was expressed as the ratio of mean cell number from invasion chamber to mean cell number from control chamber according to the manufacturer’s recommendation; B: The percentage of migration was expressed as the ratio of mean cell number in control inserts containing siRNA-treated cells to mean cell number in control inserts containing untreated control cells. Control cells were given a value of 100%.