Clinicopathological and biological significance of cripto overexpression in human colon cancer

Figure 1 Suppression of cripto mRNA by six siRNA transfection in LS-174T cells. The colon cancer cell line LS-174T was transfected with 100 nmol/L of six siRNA after 48-h incubation. Real-time polymerase chain reaction quantification of the relative amount of cripto transcript was performed using the cripto primers and probe set as described in “Materials and Methods,” using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as internal standard. All data are presented as the mean ± SD of three independent experiments; the level of cripto mRNA relative to GAPDH in untreated cells maintained under identical experimental conditions was taken as 100%.