P115 promotes growth of gastric cancer through interaction with macrophage migration inhibitory factor

Figure 6 P115 promoted the secretion and expression of MIF in culture supernatant and cells. (A) 48 h after transfection with 2 μg pEGFP-N1-P115, the secretion of migration inhibitory factor (MIF) into the supernatant was measured by ELISA. MIF in cells was increased by pEGFP-N1-P115 as measured by real-time polymerase chain reaction (PCR) (B) and Western blotting (C). β-actin was used as a loading control for Western blotting and glyceraldehyde 3-phosphate dehydrogenase was used as an internal control for PCR. Data are mean ± SD of three experiments.