P115 promotes growth of gastric cancer through interaction with macrophage migration inhibitory factor

Figure 5 P115 promoted cell proliferation and G₀-G₁ to S phase transition. (A) After transfection with 2 μg pEGFP-N1-P115 for 24, 48 and 72 h, the proliferation rate of MKN-28 cells was increased as detected by MTT assay. (B) MKN-28 cells were transfected with 2 μg pEGFP-N1-P115 for 48 h. The cell cycle was then measured by flow cytometry (B) and cell cycle regulators were measured by Western blotting (C). β-actin was used as a control for sample loading. Data are mean ± SD of three experiments.