P115 promotes growth of gastric cancer through interaction with macrophage migration inhibitory factor

Figure 3 P115-shRNA inhibited cell proliferation and G₀-G₁ to S phase transition. A: After transfection with 2 μg P115-shRNA for 24, 48 and 72 h, the proliferation rate of BGC-823 cells was inhibited as detected by MTT assay; B: BGC-823 cells were transfected with 2 μg P115-shRNA for 48 h. The cell cycle was then measured by flow cytometry (B) and cell cycle regulators were measured by Western blotting (C). β-actin was used as a control for sample loading.