Silencing Bmi-1 enhances the senescence and decreases the metastasis of human gastric cancer cells

Table 1 β-Gal activity assay and Boyden chamber assay to investigate the effects of Bmi-1 on cell senescence and metastasis (n = 5, mean ± SD)

Group	Transfected plasmids	β-Gal activity assay	Boyden chamber assay (cell number)
1	pRNAT-U6.2-si1104	28.3% ± 3.9%b	22.4 ± 4.2b
2	pRNAT-U6.2-si1356	25.9% ± 4.3%	33.6 ± 5.5b
3	pRNAT-U6.2	15.6% ± 2.7%	74.7 ± 9.3
4	Non-transfected	17.2% ± 3.1%	68.9 ± 10.1

Group 1: Transfected BGC823 cells with pRNAT-U6.2-si1104; Group 2: Transfected BGC823 cells with pRNAT-U6.2-si1356; Group 3: Transfected BGC823 cells with pRNAT-U6.2 (empty vector); Group 4: Non-transfected BGC823cells (blank).

^bP < 0.01 vs non-transfected and transfected BGC823 cells with empty vector pRNAT-U6.2.