Expression of hepatitis B virus 1.3-fold genome plasmid in an SV40 T-antigen-immortalized mouse hepatic cell line

Figure 5 Analysis of hepatitis B surface antigen and hepatitis B c antigen expression in hepatitis B virus 1. 3-fold genome plasmids-transfected cells by immunofluorescence microscopy (× 200). A: Hepatitis B surface antigen (HBsAg) observed in the hepatitis B virus 1.3-fold genome plasmids (pHBV1.3)- transfected cells at 24 h post-transfection; B: Hepatitis B c antigen (HBcAg) observed in the pHBV1.3-transfected cells at 24 h post-transfection; C: HBsAg observed in HepG2.215 cells (positive control); D: HBsAg observed in untransfected SV40 T-antigen (SV40T)-immortalized cells (negative control); E: HBcAg observed in HepG2.215 cells (positive control); F: HBcAg observed in untransfected SV40T-immortalized cells (negative control).