Amelioration of carbon tetrachloride-induced cirrhosis and portal hypertension in rat using adenoviral gene transfer of Akt

Figure 5 Western blotting analysis for apoptotic mediators and activation of hepatic stellate cells and endothelial nitric oxide synthase level. A: Apoptotic mediators and activation of HSC; Liver tissue from each group of rats were homogenized and subjected to measurement for the levels of Akt, phosphorylated Akt (p-Akt), Fas, Caspase-9 and Caspase-3, and HSC activation markers, DR5 and α-SMA, as designated in the figure. B: eNOS level; Liver tissues from each group of rats were homogenized and subjected to the measurements for Akt, pAkt, eNOS and phosphorylated eNOS (p-eNOS). Blotting of HA was used to confirm the expression of recombinant HA-Akt protein, and β-actin was used for the loading control. Data are representative of 3 experiments. HSC: Hepatic stellate cells; eNOS: Endothelial nitric oxide synthase.