Amelioration of carbon tetrachloride-induced cirrhosis and portal hypertension in rat using adenoviral gene transfer of Akt

Figure 4 Flow cytometry analysis for hepatocyte apoptosis in each experimental group. Single hepatocytes from each experiment groups were prepared at 2 wk after CCl₄ treatment, and subjected to double staining of Propidium iodide (PI) and Annenin V to detect the dead and apoptotic cell. Representative data from each group are shown with designated name. Double positive cell with PI and Annenin V were considered as apoptotic dead cells and its percentage is listed in the right upper quadrant of the plot.