Events associated with apoptotic effect of p-Coumaric acid in HCT-15 colon cancer cells

Figure 5 Apoptosis evaluation using Yo-Pro-1 dye by flow cytometry. Human colorectal carcinoma (HCT-15) cells were treated with p-Coumaric acid for specified time points. The distribution of cell population changed according to the exposure time as indicated by M1 and M2. Percentage of M2 population depicting apoptosis increased on the basis of the duration of treatment. Data is representative of three independent experiments and the differences in the values of M2 were significant at 24 and 48 h (P < 0.05 vs untreated control cells) compared to untreated control cells.