8-bromo-7-methoxychrysin inhibits properties of liver cancer stem cells via downregulation of β-catenin

Figure 1 Isolation and characterization of liver cancer stem cells derived from the MHCC97 cell line. A: Flow cytometry analysis of CD133 expression following sorting. CD133⁺ cells from MHCC97 cells formed liver cancer spheroids in stem cell-conditioned medium (200 × magnification); B: Anchorage-dependent growth of MHCC97 cells, tumor spheroid formed by CD133⁺ cells, tumor spheroid formed by parental MHCC97 cells; C: Secondary tumorspheres formed by single cells from dissociated primary liver spheroids (400 × magnification); D: Expression of stem cell surface markers CD44 and CD133 in CD133⁺ sphere-forming cells (SFCs) and parental cells; E: Hematoxylin-eosin staining revealed similar histological characteristics in tumor xenografts derived from CD133⁺ SFCs and their parental cells (100 × magnification).