1,3-Bis(2-chloroethyl)-1-nitrosourea enhances the inhibitory effect of Resveratrol on 5-fluorouracil sensitive/resistant colon cancer cells

Figure 3 Combined effects of 1,3-Bis(2-chloroethyl)-1-nitrosourea and resveratrol on various cellular markers in HCT-116 colon cancer cells. Expression pattern of apoptotic, DNA damage/repair and cell cycle regulatory proteins after drug treatment. Data are the representation of one of the replicates of three different experiments. Glyceraldehyde phosphate dehydrogenase (GAPDH) served as a loading control. BAX: Bcl-2-associated X protein; BCL-XL: B-cell lymphoma-extra large; PARP: Poly (ADP-ribose) polymerase; PCNA: Proliferating cell nuclear antigen; PTEN: Phosphatase and tensin homolog; FEN1: Flap endonuclease 1; CDC-2: Cyclin dependent kinase-1; Res: Resveratrol; BCNU: 1,3-Bis(2-chloroethyl)-1-nitrosourea; APE: Apurinic/apyrimidinic (AP) endonuclease.