Association between Ras association domain family 1A promoter methylation and hepatocellular carcinoma: A meta-analysis

Table 2 Primer sequences used in included studies for detection of Ras association domain family 1A methylation

Author	Year	Method	Forward	Reverse	Annealing T (°C)
Chan et al[22]	2008	MSRE-qPCR	5'-AGCCTGAGCTCATTGAGCTG-3'	5'--ACCAGCTGCCGTGTGG-3'	60
Chang et al[23]	2008	MSP	M 5'-GTGTTAACGCGTTGCGTTGCGTATC-3'	M 5'-AACCCCGCGAACTAAAAACGA-3'	60
			U 5'-TTTGGTTGGAGTGTGTTAATGTG-3'	U 5'-CAAACCCCACAAACTAAAAACAA-3'
Maurizio et al[24]	2011	MSP	NA	NA	NA
Mohamed et al[25]	2012	QMSP	5'-AGCCTGAGCTCATTGAGCTG-3'	5'-ACCAGCTGCCGTGTGG-3'	60
Azab et al[26]	2011	MSP	M 5'-GTGTTAACGCGTTGCGTATC-3';	M 5'-AACCCCGCGAACTAAAAACGA-3';	54
			U 5'-TTTGGTTGGAGTGTGTTAATGTG-3'	U 5'-CAAACCCCACAAACTAAAAACAA-3'
Zhang et al[27]	2007	MSP	M 5'-GTGTTAACGCGTTGCGTATC-3'	M 5'-AACCCCGCGAACTAAAAACGA-3'	60
			U 5'-TTTGGTTGGAGTGTGTTAATGTG-3'	U 5'-CAAACCCCACAAACTAAAAACAA-3'
Huang et al[28]	2011	MSRE-qPCR	5'-AGCCTGAGCTCATTGAGCTG-3'	5'-ACCAGCTGCCGTGTGG-3'	58

M: Methylated sequence; NA: Not available; U: Unmethylated sequence; MSP: Methylation-specific polymerase chain reaction (PCR); QMSP: Quantitative methylation-specific PCR; MSRE-qPCR: Methylation-sensitive endonuclease-qPCR.