Reference genes for quantitative RT-PCR data in gastric tissues and cell lines

Table 3 Ranking of the candidate single reference genes by each software package used

	NormFinder		geNorm™		BestKeeper		DataAssist™
	Stability value1	Ranking	M value1	Ranking	Coefficient of variance1	Ranking	Score1	Ranking
Neoplastic tissues
	0.24	ACTB	0.07	GAPDH	9.78	ACTB	1.58	ACTB
	0.73	B2M	0.07	B2M	11.77	B2M	1.70	B2M
	0.99	GAPDH	0.08	ACTB	13.39	GAPDH	1.88	GAPDH
	1.41	18S rRNA	0.12	18S rRNA	17.14	18S rRNA	2.25	18S rRNA
Adjacent non-neoplastic tissues
	0.61	B2M	0.05	GAPDH	7.80	ACTB	1.65	B2M
	0.65	GAPDH	0.05	B2M	9.61	B2M	1.77	ACTB
	0.65	ACTB	0.07	ACTB	10.52	GAPDH	1.81	GAPDH
	1.53	18S rRNA	0.13	18S rRNA	16.57	18S rRNA	2.46	18S rRNA
Matched pairs of adjacent non-neoplastic and neoplastic gastric tissues
	0.14	ACTB	0.06	GAPDH	9.24	ACTB	1.83	GAPDH
	0.29	B2M	0.06	B2M	11.31	B2M	1.83	ACTB
	0.41	GAPDH	0.07	ACTB	12.52	GAPDH	2.00	B2M
	0.54	18S rRNA	0.13	18S rRNA	16.86	18S rRNA	2.60	18S rRNA
Normal gastric tissues
	0.38	GAPDH	0.06	GAPDH	7.38	18S rRNA	1.82	GAPDH
	0.41	ACTB	0.06	ACTB	11.08	GAPDH	2.24	ACTB
	1.07	B2M	0.07	B2M	11.46	ACTB	2.40	B2M
	2.24	18S rRNA	0.11	18S rRNA	13.34	B2M	3.91	18S rRNA
All gastric tissues
	0.14	ACTB	0.07	GAPDH	10.89	ACTB	2.04	ACTB
	0.36	B2M	0.07	B2M	13.12	B2M	2.09	B2M
	0.69	GAPDH	0.08	ACTB	13.24	18S rRNA	2.12	GAPDH
	0.96	18S rRNA	0.13	18S rRNA	13.43	GAPDH	3.34	18S rRNA
Cell lines
	0.53	ACTB	0.06	ACTB	2.36	18S rRNA	1.71	ACTB
	1.55	B2M	0.06	B2M	4.81	ACTB	2.45	B2M
	1.73	18S rRNA	0.13	18S rRNA	8.60	B2M	2.63	18S rRNA
All gastric tissues + cell lines
	0.45	ACTB	0.09	ACTB	10.67	ACTB	2.73	B2M
	1.11	B2M	0.09	B2M	13.02	B2M	3.32	ACTB
	1.19	18S rRNA	0.16	18S rRNA	13.41	18S rRNA	3.38	18S rRNA

¹A lower value indicates increased stability in gene expression.