Curcumin cytotoxicity is enhanced by PTEN disruption in colorectal cancer cells

Figure 4 PTEN deficiency does not enhance curcumin-induced apoptosis. A: Images showing flow cytometric analysis of apoptosis. Apoptosis was analyzed by flow cytometry with the Annexin V-FITC kit; B: Histograms showing apoptosis assay results. Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) deletion did not result in increased apoptosis following curcumin treatment; C: Western blotting analysis. Cell lysates were separated on SDS-PAGE, transferred to polyvinylidene difluoride membranes, and incubated with indicated antibodies. Cur: Curcumin; PI: Propidium iodide; FITC: Fluorescein isothiocyanate; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.