Curcumin cytotoxicity is enhanced by PTEN disruption in colorectal cancer cells

Figure 1 Gene targeting at the PTEN locus. A: Schematic illustration of the strategy used to inactivate phosphatase and tensin homolog deleted on chromosome 10 (PTEN) by somatic cell gene targeting. The primers (P1, P2, P3, P4, P5, and P6) used for polymerase chain reaction (PCR)-based genotyping are indicated; B: Identification of the desired targeted clones by PCR. Primers P5 and P6 were used to amplify a 1,155-bp product from the wild-type PTEN allele and a 522-bp product from the final knockout allele, respectively; C: Western blotting analysis of PTEN expression; D: Confirmation of PTEN exon 4 deletion in PTEN-deficient cells by sequencing; E: Morphology of PTEN^+/+ and PTEN^-/- cells. F: Determination of cell viability for PTEN^+/+ and PTEN^-/- cell lines using the MTT assay. PTEN^-/-1# and PTEN^-/-2# are two PTEN knockout clones. LA: Left arm; RA: Right arm; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.