Isolation and biochemical analysis of vesicles from taurohyodeoxycholic acid-infused isolated perfused rat livers

Figure 3 A typical high-performance liquid chromatography chromatogram of a liver homogenate. Phospholipids were separated by thin layer chromatography (TLC) and the phosphatidylcholine band was scraped off the TLC plate and extracted with 2 × 1 mL methanol [high-performance liquid chromatography (HPLC) grade]. After determination of the phospholipid, 20 nmol was injected onto HPLC for the analysis of the fatty acid composition of the phosphatidylcholine as described in the methods section. Results are presented as mean ± SE (n = 6). A typical chromatogram is presented. Peak 1: Not identified; peak 2: 1-palmitoyl 2-arachidonyl (16:0-20:4) phosphatidylcholine; peak 3: 1-palmitoyl 2-linoleyl (16:0-18:2) phosphatidylcholine; peak 4: 1-palmitoyl 2-oleoyl (16:0-18:1) phosphatidylcholine; peak 5: 1-stearol 2-arachidonyl (18:0-20:4) phosphatidylcholine.