Isolation and biochemical analysis of vesicles from taurohyodeoxycholic acid-infused isolated perfused rat livers

Figure 1 Total phospholipids, cholesterol, protein and phosphodiesterase I activity in subcellular fractions of liver homogenate. A: Phospholipids; B: Cholesterol; C: Protein; D: Phosphodiesterase I activity (PDE). THDC: Taurohyodeoxycholic acid. Symbols are homogenate (H), supernatant 1 (S1), pellet 1 (P1), supernatant 2 (S2), pellet 2 (P2), supernatant 3 (S3), pellet 3 (P3) and pellet 3 (P3II) diluted with sucrose and KCI. Results are presented as mean ± SE (n = 6). Significant differences from controls were assessed by student’s t-test and are indicated by (^aP < 0.05 vs control group).