Electroacupuncture improves gut barrier dysfunction in prolonged hemorrhagic shock rats through vagus anti-inflammatory mechanism

Figure 7 Intestinal ZO-1 protein expression at 12 h after blood loss. Intestinal extracts were obtained from animals at 12 h after blood loss for measurement of ZO-1 protein expression using Western blotting. Representative Western blotting for the ZO-1 protein is shown with its corresponding glyceraldehyde 3-phosphate dehydrogenase loading control to demonstrate equal protein load in all lanes. Electroacupuncture at ST36 resulted in preservation of protein expression. Significant reduction in ZO-1 expression was seen in all the other groups. ^aP < 0.05 vs EAN group, (3-5 animals per group at 12h after blood loss). EA: Electroacupuncture; VGX: Vagotomy; α-BGT: α-bungarotoxin; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.