Aberrant TGF-&beta;1 signaling contributes to the development of primary biliary cirrhosis in murine model

doi:10.3748/wjg.v19.i35.5828

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Sep 21, 2013; 19(35): 5828-5836
Published online Sep 21, 2013. doi: 10.3748/wjg.v19.i35.5828

Table 1 Primers for real-time polymerase chain reaction

Gene	Genbank no	Forward primer (5' to 3')	Reverse primer (5' to 3')
TGFβ1	NM_011577	TGCTAATGGTGGACCGCAA	CACTGCTTCCCGAATGTCTGA
TβRI	NM_009370	ATGGTTCCGAGAGGCAGAGAT	CCATGTCCCATTGTCTTTGTTG
TβRII	NM_009371	CCAGAAGTCCTGCATGAGCAA	TGGCAAACCGTCTCCAGAGTA
Smad2	NM_010754	TCTCCGGCTGAACTGTCTCCTA	GCGATTGAACACCAGAATGCA
Smad3	NM_016769	ATGGAGCTCTGTGAGTTTGCCT	TGGAGGTAGAACTGGCGTCTCT
α-SMA	NM_007392	CTATTCAGGCTGTGCTGTCCCT	GCCCTCATAGATAGGCACGTTG
α1(I) collagen	NM_007742	CCCAAGGAAAAGAAGCACGTC	AGGTCAGCTGGATAGCGACATC
GAPDH	NM_008084	AGCCTCGTCCCGTAGACAAAA	TGGCAACAATCTCCACTTTGC

TGF: Transforming growth factor; TβR: TGF-β receptor; SMA: Smooth muscle actin; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.

Citation: Liu B, Zhang X, Zhang FC, Zong JB, Zhang W, Zhao Y. Aberrant TGF-β1 signaling contributes to the development of primary biliary cirrhosis in murine model. World J Gastroenterol 2013; 19(35): 5828-5836
URL: https://www.wjgnet.com/1007-9327/full/v19/i35/5828.htm
DOI: https://dx.doi.org/10.3748/wjg.v19.i35.5828