Clonal immunoglobulin heavy chain and T-cell receptor γ gene rearrangements in primary gastric lymphoma

Table 1 Primer sequences

Gene	Sequence
IgH tube A
VH1-FR1	5’GGCCTCAGTGAAGGTCTCCTGCAAG3’
VH2-FR1	5’GTCTGGTCCTACGCTGGTGAAACCC3’
VH3-FR1	5’CTGGGGGGTCCCTGAGACTCTCCTG3’
VH4-FR1	5’CTTCGGAGACCCTGCCCTCACCTG3’
VH5-FR1	5’CGGGGAGTCTCTGAAGATCTCCTGT3’
VH6-FR1	5’TCGCAGACCCTCTCACTCACCTGTG3’
JH consensus	5'CTTACCTGAGGAGACGGTGACC3'
IgH tube B
VH1-FR2	5’CTGGG TGCGA CAGGC CCCTG GACAA3’
VH2-FR2	5’TGGAT CCGTC AGCCC CCAGG GAAGG3’
VH3-FR2	5’GGTCC GCCAG GCTCC AGGGA A3’
VH4-FR2	5’TGGAT CCGCC AGCCC CCAGG GAAGG3’
VH5-FR2	5’GGGTG CGCCA GATGC CCGGG AAAGG3’
VH6-FR2	5’TGGAT CAGGC AGTCC CCATC GAGAG3’
VH7-FR2	5’TTGGG TGCGA CAGGC CCCTG GACAA3’
JH consensus	5'CTTACCTGAGGAGACGGTGACC3'
IgH tube C
VH1-FR3	5’TGGAG CTGAG CAGCC TGAGA TCTGA3’
VH2-FR3	5’CAATG ACCAA CATGG ACCCT GTGGA3’
VH3-FR3	5’TCTGC AAATG AACAG CCTGA GAGCC3’
VH4-FR3	5’GAGCT CTGTG ACCGC CGCGG ACACG3’
VH5-FR3	5’CAGCA CCGCC TACCT GCAGT GGAGC3’
VH6-FR3	5’GTTCT CCCTG CAGCT GAACT GTGTG3’
VH7-FR3	5’CAGCA CGGCA TATCT GCAGA TCAG3’
JH consensus	5'CTTACCTGAGGAGACGGTGACC3'
TCR-γ tube A
Vγ1f	5’GGAAG GCCCC ACAGC RTCTT3’
vγ10	5’AGCATGGGTAAGACAAGCAA3’
Jγ1.1/2.1	5’TTACCAGGCGAAGTTACTATGAGC3’
Jγ:1.3/2.3	5’GTGTTGTTCCACTGCCAAAGAG3’
TCR-γ tube B
Vγ9	5’CGGCA CTGTC AGAAA GGAATC3’
Vγ11	5’CTTCC ACTTC CACTT TGAAA3’
Jγ1.1/2.1	5’TTACCAGGCGAAGTTACTATGAGC3’
Jγ:1.3/2.3	5’GTGTTGTTCCACTGCCAAAGAG3’

The detection of immunoglobulin heavy chain (IgH) gene rearrangement using three sets of VH primers and one JH consensus primer combined in three multiplex tubes. The detection of T-cell receptor γ (TCR-γ) gene rearrangement using four Vγ primers and two Jγ primers, which were divided into two tubes.