Hypermethylation of TGF-β1 gene promoter in gastric cancer

Figure 1 Detection of transforming growth factor-β1 promoter methylation by methylation-specific polymerase chain reaction. Genomic DNA was extracted from human gastric tissues. A: Representative methylation-specific polymerase chain reaction results from gastric cancer (GC) tissues with or without Helicobacter pylori (H. pylori) infection are shown; B: Levels of transforming growth factor-β1 (TGF-β1) promoter methylation in GC tissues, non-cancerous gastric mucosa from the GC patients (GC, non-tumor) and normal gastric mucosa from non-GC subjects (Non-GC tissues); C: Impact of H. pylori status on the levels of TGF-β1 promoter methylation in GC tissues (GC, tumor), non-cancerous gastric mucosa from the GC patients (GC, non-tumor) and combined samples (overall). HP+: H. pylori-positive; HP-: H. pylori-negative; U: Unmethylated; M: Methylated.