Investigation of genome instability in patients with non-alcoholic steatohepatitis

Table 2 Total numbers and frequencies of binucleated cells with micronucleus, nucleoplasmic bridges and nuclear buds scored using the cytokinesis-blocked micronucleus cytome assay in phytohemagglutinin-stimulated lymphocytes from patients with non-alcoholic steatohepatitis and the control subjects

ID	Age (yr)	Sex	No. of MN in BN cells1	Distribution of BN cells with					No. of BN cells with NPBs	No. of BN cells with NBUDs
				1MN	2MN	3MN	4MN	5MN
Patients with non-alcoholic steatohepatitis
1	45	M	19	15	-	-	1	-	25	19
2	60	M	24	19	1	1	-	-	46	11
3	50	M	33	24	1	1	2	-	33	22
4	66	M	41	39	1	-	-	-	20	22
5	22	M	8	8	-	-	-	-	32	17
6	43	M	33	29	2	-	-	-	25	14
7	36	F	14	14	-	-	-	-	21	17
8	51	F	9	9	-	-	-	-	16	10
9	35	M	21	16	-	-	-	1	25	14
10	42	M	11	11	-	-	-	-	12	15
11	30	F	28	24	2	-	-	-	50	20
12	22	M	11	7	2	-	-	-	17	15
13	31	M	12	10	1	-	-	-	21	16
14	41	M	15	13	1	-	-	-	18	25
15	33	M	10	10	-	-	-	-	30	10
16	44	F	22	17	1	1	-	-	58	21
17	47	M	20	13	2	1	-	-	40	17
18	20	M	25	16	1	1	-	1	14	10
19	22	M	24	20	-	-	1	-	32	8
20	46	F	37	27	2	2	-	-	25	10
21	68	F	36	26	5	-	-	-	63	28
22	48	M	17	15	1	-	-	-	28	6
23	60	M	22	18	-	-	1	-	36	19
24	45	F	24	20	2	-	-	-	25	14
25	41	M	24	19	1	1	-	-	20	10
The control subjects
1	45	M	5	5	-	-	-		11	3
2	60	M	8	6	1	-	-		10	5
3	50	M	7	7	-	-	-		6	5
4	66	M	11	9	1	-	-		12	6
5	22	M	2	2	-	-	-		2	4
6	43	M	6	4	1	-	-		1	4
7	36	F	3	3	-	-	-		9	4
8	51	F	9	5	2	-	-		5	3
9	35	M	4	2	1	-	-		4	1
10	42	M	6	6	-	-	-		7	5
11	30	F	8	8	-	-	-		1	4
12	22	M	2	2	-	-	-		8	4
13	31	M	2	2	-	-	-		10	5
14	41	M	7	5	1	-	-		16	8
15	33	M	7	7	-	-	-		12	6
16	44	F	11	8	-	1	-		5	6
17	47	M	9	7	1	-	-		4	2
18	20	M	1	1	-	-	-		8	4
19	22	M	4	4	-	-	-		9	1
20	46	F	11	7	2	-	-		8	3
21	65	F	17	15	1	-	-		9	5
22	48	M	12	10	1	-	-		14	6
23	60	M	6	6	-	-	-		13	3
24	45	F	11	9	1	-	-		5	3
25	41	M	3	3	-	-	-		7	5

The numbers of micronucleus (MN), nucleoplasmic bridges (NPBs) and nuclear buds (NBUDs) were scored on 1000 binucleated (BN) cells per subject.

¹Total number of MN: (1MNX1) + (2MNX2) + (3MNX3) + (4MNX4) + (5MNX5). M: Male; F: Female.