Analysis of single nucleotide polymorphisms in the region of CLDN2-MORC4 in relation to inflammatory bowel disease

Table 3 Primers for polymerase chain reaction amplification and sequencing of Claudin-2

Candidate gene	Exon	Forward primer1	Reverse primer2	Size of PCR-product
CLDN2	1	GTAGGACCTGCTCTTTGAACTC23	TGAATTTAAAAGGCAGCAACTA23	708 bp
	1	TCAATCTTTCCCAGCCTCCA3	TTTCTGTCAAAAACTCCACTCC3
	2	TTGTAGAGAATGGGGAGGTGTGC23	TCAATTGCAGACTGAGGCCAAA2	599 bp
	2		TGCAGACTGAGGCCAAACTG3
	2	CTAGGCCCCTGGAGATTCAAGA23	TGTGCCCAAAAGCCCCAGAA23	682 bp
	2	TTCCTTTCTCATGTGTTATTTCTAA3	GAGAAAGGAAAAAAAAAACAAC3
	2	TGAGGGATTAGAGGTGTTCAA23	GCAGCACCTTCTGACACGA23	892 bp
	2	ATCCTACGGGACTTCTACTCA3	ACTCCACCTGCTACCGCCACT3

¹q, Q-solution for polymerase chain reaction (PCR)-amplification;

²Primer used for PCR;

³Primer used for sequencing. CLDN: Claudin.