Mesenchymal stem cells alleviate TNBS-induced colitis by modulating inflammatory and autoimmune responses

doi:10.3748/wjg.v19.i29.4702

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Aug 7, 2013; 19(29): 4702-4717
Published online Aug 7, 2013. doi: 10.3748/wjg.v19.i29.4702

Table 1 Sequence of gene-specific primers used for real-time polymerase chain reaction analysis

mRNA target	Forward primer sequences 5’→3’	Reverse primer sequences 5’→3’	bp
Actin	CGTTGACATCCGTAAAGACC	CTAGGAGCCAGAGCAGTAATC	111
IL-2	TGAGTGCCAATTCGATGATGAG	TTATTGAGGGCTTGTTGAGATGAT	91
IL-4	CAGCAACGAAGAACACCACAG	CGAAAAGCCCGAAAGAGTC	148
IL-6	AATTTCCTCTGGTCTTCTGG	ACTCTGGCTTTGTCTTTCTTGTT	93
IL-10	AGTGGAGCAGGTGAAGAGTGATTT	CTATGCAGTTGATGAAGATGTC	92
IL-17A	GAGAGCTGCCCCTTCACTTTCA	GGCTGCCTGGCGGACAAT	87
IFN-γ	TGGTGACATGAAAATCCTG	TTGCTGTTGCTGAAGAAG	141
TNF-α	AGTTCCCAAATGGCCTCCCT	ACTTGGTGGTTTGCTACGAC	115
GATA3	AGGTGCATGACGCGCTGGAG	GGAGTGGCTGAAGGGAGAG	107
Foxp3	GAAGAATGCCATCCGCCACAA	TGCTCCCTTCTCGCTCTCCA	70
TGF-β	TGCCCTCTACAACCAACACAAC	GCAGGAGCGCACAATCAT	142
T-bet	ACCTCTTCTATCCAACCAGTATCC	GAGGTGTCCCCAGCCAGTA	104
RORγt	GAAGGCAAATACGGTGGTGTGG	GCTGAGGAAGTGGGAAAAGTC	90

IL: Interleukin; IFN: Interferon; TNF: Tumor necrosis factor; GATA3: GATA family of transcription factors 3; Foxp3: Forkhead box P3; TGF: Transforming growth factor; T-bet: T-box expressed in T cells; RORγt: Retinoid related orphan receptor gamma(t).

Citation: Chen QQ, Yan L, Wang CZ, Wang WH, Shi H, Su BB, Zeng QH, Du HT, Wan J. Mesenchymal stem cells alleviate TNBS-induced colitis by modulating inflammatory and autoimmune responses. World J Gastroenterol 2013; 19(29): 4702-4717
URL: https://www.wjgnet.com/1007-9327/full/v19/i29/4702.htm
DOI: https://dx.doi.org/10.3748/wjg.v19.i29.4702